CBD products are made from high-CBD hemp flowers, the same species of plant as marijuana. So the legal regulations of CBD have a lot of confusion surrounding them..
Many first time consumers of CBD products are intimidated by all the new terms and don’t know where to start. This article will answer one of the most popular questions: what is the difference between a CBD tincture and a CBD concentrate?
Thanks to relatively small doses, you can administer CBD tincture in many different ways. You can easily add it to drinks like coffee and tea. If you are looking to take your CBD with food, mix a tincture into a meal like soup or pasta.
CBD tinctures containing more than 0.3 percent THC are classified as a Class I drug and, therefore, are federally illegal. However, most CBD tinctures are extracted from the hemp plant. So they have plenty of cannabidiol and low THC concentration. Regardless of THC concentration, the sale and consumption of all CBD tinctures is only legal in states that legalized recreational and medical marijuana.
What Is the Difference Between CBD Concentrate and CBD Tincture?
CBD tinctures come in small to medium size tinted glass bottles. Tinted bottles extend the contents’ shelf life, plus they block UV light, keeping CBD products fresh. CBD tinctures are very concentrated, so you have to take them in small doses. That’s why every bottle comes with a dropper. It helps you get the dosing right and makes the consumption of cannabidiol hassle-free.
Tinctures contain additional ingredients, such as flavorings. So we do not recommend applying them topically on the skin. Sometimes it can lead to irritation.
The main difference between CBD Tinctures and CBD Concentrate is how much CBD is in each mL of liquid. nu-x® CBD Concentrate is available at 3,000mg of CBD per 30 mL bottle which is more CBD per mL than the nu-x® CBD Tincture, which is available with 1,000mg of CBD per 30mL bottle.
It is possible to take tinctures sublingually or by applying them underneath the tongue. This method of delivering CBD tincture is common.
You can consume your CBD concentrates in several ways. From smoking them along with a CBD flower, vaporizing using a vape pen or standalone vaporizer, smoking dabs using a dab rig, to even adding them into your food or drink.
HeLa-luc cells are stably transfected with a luciferase reporter gene controlled by the interleukin (IL-6) promoter. The plasmid upstream from a luciferase gene, together with the selection plasmid pMEP4 includes the recombination signal sequence binding protein Jkappa, which is constitutively bound to the NF-κB site of the IL-6 promoter and acts as a negative regulatory factor. IL-6 is one of the target genes for activated NF-κB. Therefore, the luciferase produced can be measured by IL-6 dependently, signalling activation or inhibition of NF-κB. Tests were performed as previously described . In brief, cells were exposed to tincture samples (final concentrations in the media 0.002–25 μL/mL) for 40 min before the stimulant phorbol myristate acetate (PMA, 50 ng/mL) was added. The cells were incubated (37 °C) for 6 h before lysis, transfer, and automatic addition of the luciferase substrate (Promega, Southampton, UK) using a luminometer/photometer (Anthos Lucy 1) and the resulting luminometric reading recorded following a reaction time of 10 s. Positive (stimulated cells without sample) and negative (resting cells without stimulation) controls were included. Means were calculated from two readings per sample, two samples per plate, two plates per experiment, and two independent experiments. SEMs were calculated for the latter two (n = 4). Tincture samples were tested when fresh and after 6 months of storage at −18 °C in the dark.
If the starting material consists strictly of female flowering tops, traditional tinctures using a minimum of 60% EtOH had a cannabinoid content > 6 mg/mL with THCtot representing 70%–95% of the detected cannabinoids. The ratio to the main co-cannabinoids (CBGtot) to other cannabinoids, and to flavonoids was around 10:1, respectively. Therefore, any traditional use may be primarily linked to the presence of THCA, THC, and eventually their degradation compounds including CBN. This predominance of THCtot is diminished by higher leaf portions in the drug, more polar extraction (e.g., 20% or 40% EtOH) as well as the use of ‘old’ drugs and tinctures. Cannabinoid and phenolic co-constituents may then influence overall effects: in a 40% leaf tincture the CANtot/TPC ratio was only 0.2; in a 15-month-old 60% flower tincture, CBGtot was half as concentrated as THCtot representing more than 20% of all cannabinoids. Overall, cannabinoids and cannflavins were best extracted with 60%–90% EtOH from flos; other phenolics with 60% EtOH were from folium. Due to comparable polarity, cannflavins remained in their natural subordinated proportion versus cannabinoids (maximum around 1:50 in 60% EtOH tinctures from the flowers). Cannflavins were relatively stable in tinctures, although after 15 months additional flavonoid peaks appeared in line with observations from the reference substances as described under “Experimental”.
2.5.3. Group and Ratio Marker Calculation
Two methods previously described and validated were applied . The overall fingerprint (detection at 214, 254, 275, and 324 nm) allowed us to address the ratio between cannabinoids (CANtot) and other phenolic compounds (TPC), while the cannabinoid profile method (λ = 214 nm) served for the quantification of cannabinoids and cannflavins. The HPLC Waters™ system 900, with a Waters™ 996 PDA detector and a Waters™ 717 plus autosampler device and Millenium or EmPower software were used equipped with an Ace ® 5 Phenyl (25 cm × 4.6 mm) column (ACT, Aberdeen, UK) for the fingerprint and an Agilent Zorbax RX-C18 column (5 µm 4.6 × 250 mm Highchrom, Reading, UK) and a Nova-Pak ® C8 Guard Column 3.9 × 20 mm, 2/pkg (Waters UK Elstree, UK) for the cannabinoid profile. Gradient solvent mixtures of water (TFA 0.1%) (solvent A), a water-acetonitrile mixture (65:35, TFA 0.1%) (solvent B), and acetonitrile (solvent C) were used for the fingerprint (80 min including pre- and washing phase). Solvent B and C were used for the cannabinoid profile (55 min including pre- and washing phase). Reference standards were used as described previously, while liquid extracts were injected directly or after 1:1 dilution, in case of highly concentrated tinctures.
Special thanks to Jose Maria Prieto (School of Pharmacy, London) for the scientific and technical support as well as Elizabeth Williamson (University Reading), Keith Helliwell (Ransom, Hitchin, UK), Michael Heinrich, and Andrew Constanti for the possibility to do this work and use the facilities at the School of Pharmacy, London, the organizational framework of the European research project (COOP-CT-2004-512696) and funding by Ransom. The work was carried out between January 2008 and September 2009 with initial results included in my Ph.D. thesis (02/2009). Essential for this work was the isolation and generous provision of cannflavin A and B by the group of Giovanni Appendino (Novara, IT).
4.1. Chemical Pattern of Tinctures
Comparison of tincture profiles from Cannabis flos vs. folium. (A,B): Relative cannabinoid profile of 40% and 90% tinctures (Δ9-tetrahydrocannabinolic acid A THCA, Δ9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabigerolic acid (CBGA), cannabigerol (CBG), other non-identified cannabinoids oCAN, and cannflavins CFL after 2 days, 3 months (15–25 °C/light), 3, 9, and 15 months (4 °C/dark) storage; (C) Absolute values for THCA, THC, CBN, and their sum (THCtot) in 60% tinctures from flowering tops and leaves (ground) over 15 months (4 °C/dark). Mean of measurement in triplicate ± SD. Cannabidiol (CBD); cannabidiolic acid (CBDA).